Sugar cane bagasse pretreatment: An attempt to enhance the production potential of cellulases by Humicola insolens TAS-13

Pretreatment of the cellulosic substrate has miracle effect on the enhancement of cellulase production by fungal strains. A thermophilic strain of Humicola insolens TAS-13 was locally isolated and was tested for cellulases production under solid-state fermentation conditions using sugar cane bagasse as substrate. The cultural conditions for the H. insolens were also optimized for the higher rate of cellulase secretion. In order to enhance the production rate of heterogenouscellulosic proteins, bagasse was pretreated with NaOH, H2SO4, H2O2 and H2O2+1.5%NaOH. The pretreatment of bagasse with 2.0% H2O2 along with 1.5% NaOH enhanced the biosynthesis of cellulases by H. insolens. Production rate was also optimized with different parameters like thickness of fermentation medium, initial pH, incubation time and temperature. The thickness of the fermentation medium of 0.8 cm (10 g) with pH range of 5.5 was found to be better for enhanced production at 50°C. The yield of the enzyme was reached maximum with CMC-ase (18.98 U/g/min), FP-ase (13.63 U/g/min), -glucosidase (19.54 U/g/min) 72 h after inoculation

Ever-increasing Caesarean section and its economic burden in Bangladesh

BACKGROUND: Cesarean Section (CS) delivery has been increasing rapidly worldwide and Bangladesh is no exception. In Bangladesh, the CS rate has increased from about 3% in 2000 to about 24% in 2014. This study examines trend in CS in Bangladesh over the last fifteen years and implications of this increasing CS rates on health care expenditures. METHODS: Birth data from Bangladesh Demographic and Health Survey (BDHS) for the years 2000–2014 have been used for the trend analysis and 2010 Bangladesh Maternal Mortality Survey (BMMS) data were used for estimating health care expenditure associated with CS. RESULTS: Although the share of institutional deliveries increased four times over the years 2000 to 2014, the CS deliveries increased eightfold. In 2000, only 33% of institutional deliveries were conducted through CS and the rate increased to 63% in 2014. Average medical care expenditure for a CS delivery in Bangladesh was about BDT 22,085 (USD 276) in 2010 while the cost of a normal delivery was BDT 3,565 (USD 45). Health care expenditure due to CS deliveries accounted for about 66.5% of total expenditure on all deliveries in Bangladesh in 2010. About 10.3% of Total Health Expenditure (THE) in 2010 was due to delivery costs, while CS costs contribute to 6.9% of THE and rapid increase in CS deliveries will mean that delivering babies will represent even a higher proportion of THE in the future despite declining crude birth rate. CONCLUSION: High CS delivery rate and the negative health outcomes associated with the procedure on mothers and child births incur huge economic burden on the families. This is creating inappropriate allocation of scarce resources in the poor economy like Bangladesh. Therefore it is important to control this unnecessary CS practices by the health providers by introducing litigation and special guidelines in the health policy

Caffeic acid phenethyl ester is protective in experimental ulcerative colitis via reduction in levels of pro-inflammatory mediators and enhancement of epithelial barrier function

BACKGROUND: Inhibition of the nuclear factor kappa beta (NF-κβ) pathway has been proposed as a therapeutic target due to its key role in the expression of pro-inflammatory genes, including pro-inflammatory cytokines, chemokines, and adhesion molecules. Caffeic acid phenethyl ester (CAPE) is a naturally occurring anti-inflammatory agent, found in propolis, and has been reported as a specific inhibitor of NF-κβ. However, the impact of CAPE on levels of myeloperoxidases (MPO) and pro-inflammatory cytokines during inflammation is not clear. The aims of this study were to investigate the protective efficacy of CAPE in the mouse model of colitis and determine its effect on MPO activity, pro-inflammatory cytokines levels, and intestinal permeability. METHOD: Dextran sulphate sodium was administered in drinking water to induce colitis in C57/BL6 mice before treatment with intraperitoneal administration of CAPE (30 mg kg(-1) day(-1)). Disease activity index (DAI) score, colon length and tissue histology levels of MPO, pro-inflammatory cytokines, and intestinal permeability were observed. RESULTS: CAPE-treated mice had lower DAI and tissue inflammation scores, with improved epithelial barrier protection and significant reduction in the level of MPO and pro-inflammatory cytokines. CONCLUSION: Our results show that CAPE is effective in suppressing inflammation-triggered MPO activity and pro-inflammatory cytokines production while enhancing epithelial barrier function in experimental colitis. Thus, we conclude that CAPE could be a potential therapeutic agent for further clinical investigations for treatment of inflammatory bowel diseases in humans

Experimental characterization of gasoline sprays under highly evaporating conditions

An experimental investigation of multistream gasoline sprays under highly evaporating conditions is carried out in this paper. Temperature increase of fuel and low engine pressure could lead to flash boiling. The spray shape is normally modified significantly under flash boiling conditions. The spray plumes expansion along with reduction in the axial momentum causes the jets to merge and creates a low-pressure area below the injector’s nozzle. These effects initiate the collapse of spray cone and lead to the formation of a single jet plume or a big cluster like structure. The collapsing sprays reduces exposed surface and therefore they last longer and subsequently penetrate more. Spray plume momentum increase, jet plume reduction and spray target widening could delay or prevent the closure condition and limit the penetration (delayed formation of the cluster promotes evaporation). These spray characteristics are investigated experimentally using shadowgraphy, for five and six hole injectors, under various boundary conditions. Six hole injectors produce more collapsing sprays in comparison to five hole injector due to enhanced jet to jet interactions. The spray collapse tendency reduces with increase in injection pressure due high axial momentum of spray plumes. The spray evaporation rates of five hole injector are observed to be higher than six hole injectors. Larger spray cone angles of the six hole injectors promote less penetrating and less collapsing sprays

Growth and Production Performances of Crossbred Climbing Perch Koi, Anabas testudineus in Bangladesh

The study was performed for evaluating the growth and production performances of crossbred climbing perch (Anabas testudineus) during August to October 2009. The crossbred groups of fish were produced by crossing between native climbing perch and Thai climbing perch (A. testudineus). Growth and production performances was compared at grow out condition between crossbred groups in the two reciprocal crosses of treatments viz. T1: Native climbing perch (?) × Thai climbing perch (?) and T2: Thai climbing perch (?)×Native climbing perch (?. At the same time, they were compared also with their respective control F1 pure line groups i.e. T3: Native climbing perch (?) × Native climbing perch (?) and T4: Thai climbing perch (?) × Thai climbing perch (?). It was evident from the results that among the treatments in grow out system, the highest mean harvesting weight was 69.25±9.01 g found in T4 (Thai climbing perch), which was significantly different (P<0.05) from all other treatments. Whereas, T3 (native climbing perch) also showed the lowest mean harvesting weight 33.38±8.74 g and showed significant differences (P<0.05) among all the treatments. The mean harvesting weight of both the crossbred groups i.e. T1 and T2 were 50.83±6.65 and 59.94±7.83 g, respectively and these results were statistically insignificant (P>0.05) between them but significant among all other treatments. The highest (93.60%) and lowest (83.06%) survival rates were observed in Thai koi (T4) and native Koi (T3) parental stocks, respectively. Wider variations in terms of productions performances were observed among the treatments, where the gross production of T1, T2, T3 and T4 were 2,753±96.67, 3,401±73.26, 1,733±168.47 and 4,051±185 kg/ha, respectively. The productions obtained from crossbred groups were at intermediate compared to their control pure line groups. Higher FCR mean value was observed in native control line (T3), while, lower FCR mean value was observed in Thai control line (T4). DOI: http://dx.doi.org/10.3329/ijarit.v2i1.13990 Int. J. Agril. Res. Innov. & Tech. 2 (1): 19-25, June, 201

Design and analysis of a high-gain and robust multi-DOF electro-thermally actuated MEMS gyroscope

© 2018 by the authors. This paper presents the design and analysis of a multi degree of freedom (DOF) electro-thermally actuated non-resonant MEMS gyroscope with a 3-DOF drive mode and 1-DOF sense mode system. The 3-DOF drive mode system consists of three masses coupled together using suspension beams. The 1-DOF system consists of a single mass whose motion is decoupled from the drive mode using a decoupling frame. The gyroscope is designed to be operated in the flat region between the first two resonant peaks in drive mode, thus minimizing the effect of environmental and fabrication process variations on device performance. The high gain in the flat operational region is achieved by tuning the suspension beams stiffness. A detailed analytical model, considering the dynamics of both the electro-thermal actuator and multi-mass system, is developed. A parametric optimization is carried out, considering the microfabrication process constraints of the Metal Multi-User MEMS Processes (MetalMUMPs), to achieve high gain. The stiffness of suspension beams is optimized such that the sense mode resonant frequency lies in the flat region between the first two resonant peaks in the drive mode. The results acquired through the developed analytical model are verified with the help of 3D finite element method (FEM)-based simulations. The first three resonant frequencies in the drive mode are designed to be 2.51 kHz, 3.68 kHz, and 5.77 kHz, respectively. The sense mode resonant frequency is designed to be 3.13 kHz. At an actuation voltage of 0.2 V, the dynamically amplified drive mode gain in the sense mass is obtained to be 18.6 μm. With this gain, a capacitive change of 28.11 f F and 862.13 f F is achieved corresponding to the sense mode amplitude of 0.15 μm and 4.5 μm at atmospheric air pressure and in a vacuum, respectively

Yield Performances and Cup Quality of Some BTRI Test Clones of Tea

A long term experiment was conducted to investigate the yield and quality performances of four vegetative propagated test clones of tea coded as A/8/01, A/17/22, A/22/27 and A/22/40 at Bangladesh Tea Research Institute (BTRI) farm during 1996-2010. A standard clone BT1 was considered as control. Cuttings of the test clones were collected from the selected bushes of Amo tea estates and were raised at BTRI nursery. Then saplings were put to long term yield and quality trial following Latin Square Design (LSD) with 3 replications. The green leaf was harvested at weekly interval during the plucking season starting from mid March to mid December throughout the experimental period. Yield data were recorded and analyzed statistically using MSTAT programme. Results of the experiment revealed that among the test clones A/22/40 gave the highest significant yield of 3509.1 kg ha-1 of made tea followed by BT1 (3203.69 kg ha-1), A/8/01 (2912.24 kg ha-1), A/17/22 (2817.76 kg ha-1) and A/22/27 (2278.78 kg ha-1) from the average of 9 years (2002-2010) at mature stage. At immature stage i.e. 1st year to 5th year after plantation yield difference was insignificant. The overall cup quality of the test clones was assessed by conventional organoleptic test. The cup quality of A/8/01, A/17/22, A/22/40 as well as the standard clone BT1 was found to be above average (AA) while the cup quality of A/22/27 was average (A). Considering the yield and quality potentials, the test clone A/22/40 has appeared quite promising to be released as a standard clone. DOI: http://dx.doi.org/10.3329/ijarit.v1i1-2.13925 Int. J. Agril. Res. Innov. & Tech. 1 (1&2): 24-28, December, 201

\u27They say\u27: medical students\u27 perceptions of General Practice, experiences informing these perceptions, and their impact on career intention-a qualitative study among medical students in England

\ua9 Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.OBJECTIVES: The number of UK graduates choosing General Practice training remains significantly lower than the current numbers required to meet the demands of the service. This work aims to explore medical students\u27 perceptions of General Practice, experiences which lead to the development of these perceptions, and the ultimate impact of these on career intention. DESIGN: This mixed-methods, qualitative study used focus groups, semistructured interviews, longitudinal audio diary data and debrief interviews to explore and capture the experiences and perceptions of students in their first and penultimate years of university. SETTING: Three English medical schools. PARTICIPANTS: Twenty students were recruited to focus groups from first and fourth/fifth year of study. All students in these years of study were invited to attend. Six students were recruited into the longitudinal diary study to further explore their experiences. RESULTS: This work identified that external factors, internal driving force and the \u27they say\u27 phenomenon were all influential on the development of perceptions and ultimately career intention. External factors may be split into human or non-human influences, for example, aspirational/inspirational seniors, family, peers (human), placements and \u27the push\u27 of GP promotion (non-human). Driving force refers to internal factors, to which the student compares their experiences in an ongoing process of reflection, to understand if they feel General Practice is a career they wish to pursue. The \u27they say\u27 phenomenon refers to a passive and pervasive perception, without a known source, whereby usually negative perceptions circulate around the undergraduate community. CONCLUSION: Future strategies to recruit graduates to General Practice need to consider factors at an undergraduate level. Positive placement experiences should be maximised, while avoiding overtly \u27pushing\u27 GP onto students

Reproductive Biology of Feather Back, Chital (Notopterus chitala, Ham.) Cultured in a Pond of Bangladesh

Studies on Gonadosomatic index (GSI), ova diameter and histology of the gonad were performed to understand reproductive biology of Feather back, Chital (Notopterus chitala) for a period of 6 months from January to June 2010. Thirty live chital fish were used in this study. The mean GSI values for female chital were found to range between 0.20±0.013 and 4.63±0.50. The highest GSI value was found in June. The smallest diameter of ovum was recorded 0.04 mm (January) and the largest was 4.00 mm (June). During the experimental period, the fecundity was ranged from 8,238 to 18,569 (mean 13,052±4607) in fish samples with body weight range from 1,296 to 2,360 (mean 1,742.50±474.44 g) while the relative fecundity was 5.65 to 14.33. Histological study revealed that the percentage of late perinucleolus (LPN) stage was highest in April and Cortical alveoli (CA) stage appeared from April and reached to maximum in May. Vitellogenic stage (VG) appeared in the month of May and chronologically increased through June. Vitellogenic stage (VG) of oocyte as well as highest ova diameter reached to the peak in June. The variations in the gonad weight and GSI of the female fish reached to the peak during June indicating maturity of ovary and definite spawning season. DOI: http://dx.doi.org/10.3329/ijarit.v2i1.13991 Int. J. Agril. Res. Innov. & Tech. 2 (1): 26-31, June, 201

Effects of Stocking Density on Growth and Production Performance of Indigenous Stinging Catfish, Heteropneustes fossilis (Bloch)

An on-farm experiment was undertaken in nine earthen ponds to evaluate the growth and production potentials of stinging catfish shing, Heteropneustes fossilis for the period of six months from March to August 2010. Three stocking densities such as 1,25,000 (T1), 1,87,500 (T2) and 2,50,000 ha-1 (T3) were tested with three replications each. Fish were fed with commercial pelleted feed containing 35% crude protein. After six months rearing, the mean harvesting weights of shing were 69.42±6.20, 58.74±3.85 and 49.50±4.52g in T1, T2 and T3, respectively. Significant (P<0.05) highest mean harvesting weight was found in T1. The best survival was found in T1 (87%) among the treatments. The calculated mean production of shing (H. fossilis) in three treatments such as T1, T2 and T3 were 7549±52, 9031±71 and 8786±60 kg ha-1, respectively, which were significantly different (p<0.05) from each other. DOI: http://dx.doi.org/10.3329/ijarit.v2i2.14009 Int. J. Agril. Res. Innov. & Tech. 2 (2): 9-14 December, 201